In Vivo - non GLP contract research

     

        Pharmacokinetc studies in mice

Please note this is partly copied and pasted from a different website we need our  pictures and to modify language a bit

We offer pharmacokinetic (PK) studies in widely used murine inbred (C57BL/6, BALB/c), or custom strains. All study protocols are reviewed by our in-house Institutional Animal Care and Use Committee (IACUC). possibly link to Explora

A typical PK study in mice involves one or two drug delivery routes (e.g. PO and IV), 6 time points for each route (for example: 5, 15, 30, 60, 120, 240 min for IV and 15, 30, 60, 120, 240 and 360 min for PO) and 4 animals per  each  time  point  group/route,  plus a control  plasma of  Vehicle dosed group.  We will prepare blood plasma samples, harvest organs and ship you your samples for analysis.

We offer:

  • End-point or serial sampling;

  • Wide range of time points;

  • Native or perfused tissue sampling;

Deliverable: A detailed study report including full description of study design. Raw experimental data are available upon request.

Typical plasma concentration-time curve for single compound testing:

add refs if available

 
 

        Tail Bleeding Times

Please note this is not final

Tail bleeding times are routinely used to assess the anti-coagulation potential of a compound, or to compare coagulation efficiency in transgenic mice. The model is simple and well-described ( ref). and typical study requires 8-10 mice/ group to be completed.

 
 
 

        Ferric Chloride Thrombosis in the Left Carotid Artery

Please note this is not final

The Ferric Chloride- induced thrombosis model is a widely used technique to produce  thrombus formation in the left carotid artery of the mouse. The thrombus progression is monitored with a doppler probe that measures blood flow in the artery and injury severity can be modulated by applying different concentrations of Ferric Chloride. The vessel denudation results from free radicals generation, which causes lipid peroxidation and destruction of endothelial cells. Exposure of collagen and Tissue Factor reflects both the intrinsic and the extrinsic coagulation pathway contribution to thrombosis (insert ref). The Ferric Chloride - induced thrombosis technique allows assessment of thrombosis progression in coisogenic strains, and in isogenic mice dosed with study compounds.

 

 

 

 

 

 

 

 

 

 

 

 

 

Kurz KD, Main BW, Sandusky GE. Rat model of arterial thrombosis induced by ferric chloride. Thromb Res. 1990;60(4):269–280. 

 

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